THE ANTI-TUMOR ACTIVITIES OF PROKARYOTIC RECOMBINANT EXPRESSION PRODUCT OF GELSOLIN GENE FROM DENDRORHYNCHUS ZHEJIANGENSIS
Gelsolin is an important actin binding protein and involved in many cellular processes. In this work, anti-tumor activities of the prokaryotic recombinant expression product of gelsolin gene from Dendrorhynchus zhejiangen- sis were assessed employing the MTT assay, Hoechst33342/PI staining and a flow cytometric analysis. The results showed that the growth inhibition rate of MGC803 cells was reached to 34.53% when the cells were treated by 30~tg/ml r-gelsolin for 24h. Effects of r-gelsolin on the morphology of MGC803 cells were observed by Hoechst33342/PI staining. The cell nuclei showed pyknosis, rupture, and apoptotic bodies appeared. Furthermore, cell cycle and apoptosis were detected by flow cytometer, compared with the control group, the results showed that r-gelsolin could arrest the cells in S/G2 phase, inhibit the proliferation of cells, and induce their apoptosis.
Dendrorhynchus zhejiangensisGelsolinGastric cancer cellsCell apoptosis
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