IDENTIFICATION OF BODY-LENGTH-RELATED DART MARKERS IN NEW CALTIVAR "DONGHAI NO.1" OF LARGE YELLOW CROAKER LARIMICHTHYS CROCEA
Large yellow croaker (Larimichthys crocea) is an important marine economic fish in China.However,in recent years,many commercial traits have been degrading due to fast development of fish farming,resulting in lower growth rate,earlier sex maturation,and lower viability.Thus,the need is urgent in genetic improvement of farmed large yellow croaker.Diversity arrays technology (DArT) provides a high-throughput and low-cost whole-genome genotyping platform for the detection and scoring of hundreds of polymorphic loci without any need for prior sequence information.DArT is widely used to create genetic maps and analyze genetic diversity of animals and plants.We used DArT to identify body-length-related molecular marks of the Donghai No.1 caltivar of large yellow croaker.The body lengths of 199 fish were randomly measured and the average was 13.45cm in conformation to normal distribution.The maximal and minimal length groups were significantly different (P＜0.01).PstⅠ representations were digested with seven pairs of different frequent cutters (PstⅠ/AluⅠ,PstⅠ/BanⅡ,PstⅠ/Bsp1286I,PstⅠ/BstNI,PstⅠ/HaeⅢ,PstⅠ/RsaⅠ,PstⅠ/TaqⅠ) and used for libraries construction.PstⅠ/RsaⅠ was selected as optimal complexity reduction method according to the numbers of unique clones and polymorphism levels.An amount of 3360 PstⅠ representations as probes was amplified from the PstⅠ/RsaⅠ library for array preparation.Eighteen DArT candidate marks were screened by hybridization and data statistics.The new selected population was used to verify the candidate marks,and eight DArT markers were significantly associated with body length.These basic data highlight potential application of DArT for marker-assisted selection of large yellow croaker with good growth traits.
large yellow croaker Larimichthys croceadiversity arrays technology (DArT)body lengthgrowth trait
NETL
NSTL
万方数据
维普
