METABOLOMICS OF THE DIGESTIVE GLAND OF MYTILUS CORUSCUS UNDER HIGH-TEMPERATURE STRESS
With the continuation of global warming,the problem of ocean warming has been gradually intensified,and high temperatures seriously affect various life processes of marine organisms.However,studies on how marine bivalve mussels cope with heat stress remain insufficient.A metabolomic analysis of digestive gland tissues of M.coruscus under acute heat stress was carried out at 26 ℃ and 33 ℃ against the control at 18 ℃,to study the metabolic responses.LC-MS/MS technology was used,and differential metabolites were screened and analyzed for changes in relevant metabolic pathways.A total of 2 532 metabolites were identified in the digestive gland.KEGG enrichment analysis was used to explore potential metabolic pathways of differential metabolites,and 29 metabolic pathways were significantly enriched.Compared to the control group,the 26 ℃ group showed significant enrichment in taurine and hypotaurine metabolism,neuroactive ligand-receptor interaction,sphingolipid metabolism,and retinol metabolism,while the 33 ℃ group showed significant enrichment in the metabolisms of tyrosine,linoleic acid,alanine,tryptophan,phenylalanine,and phenylalanine.Results indicate that the digestive gland of M.coruscus responds to heat stress by mainly regulating tryptophan metabolism,tyrosine metabolism,sphingolipid metabolism,phenylalanine metabolism,oxidative phosphorylation,and degradation pathways of fatty acids and lysine to help maintain internal environmental stability.This research provided a theoretical basis for exploring the adaptative evolution of M.coruscus against temperature changes in the environment from multiple perspectives.
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