ANALYSIS OF ALTERNATIVE SPLICING EVENTS FOR INNATE IMMUNITY OF CRASSOSTREA GIGAS INDUCED BASED ON RNA-SEQ
Most invertebrates lack adaptive immunity based on classical antibodies and memory cells,thus natural immunity plays an indispensable role in invertebrate immune defense.Variable splicing is an important way to generate diversity and specificity of natural immunity.Crassostrea is a globally farmed shellfish and the most productive marine shellfish in China.Understanding the diversity and specificity of its natural immune system is essential for the prevention and control of oyster diseases and the sustainable development of oyster aquaculture.The variable splicing events in the natural immune stress response of Crassostrea gigas were investigated systematically by bioinformatic analysis of transcriptomes induced by different pathogens at different times after induction.The total number of variable splicing events was found to increase significantly under Vibrio induction,indicating that Vibrio infection induced the generation of variable splicing events in C.gigas.Functional enrichment analysis of genes with significant changes in the composition type and expression of variable spliceosomes after pathogen induction showed that immune system-related functions were significantly enriched,and that the composition type and expression of variable spliceosomes were inconsistent at different stages of pathogen infection as well as after different pathogen infections,suggesting that the immune system of the long oyster can generate specificity and diversity of immune responses through variable splicing.This study provided a typical example of the diversity and specificity of natural immunity in invertebrates such as C.gigas,and also a theoretical support for the defense of C.gigas diseases.
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