ANALYSIS OF BACTERIAL COMPOSITION AND DIVERSITY IN LOW VOLTAGE ELECTROSTATIC FIELD TREATED SOLENOCERA CRASSICORNIS BASED ON HIGH THROUGHPUT SEQUENCING
In order to obtain microbial development information and community composition changes affecting the quality of shrimp meat after electric field treatment during storage,using red shrimp Solenocera crassicornis as the experimental raw material,using high throughput sequencing technology,gene sequencing was performed on the microbial community bases of shrimp meat.Through species Wayne diagram,alpha and beta diversity,and species composition analysis,the effects of different electric field environments(2 kV,3 kV,0 kV)on the microbial community composition of S.crassicornis were studied.The results showed that the dominant bacteria in OTU cluster analysis were classified as Pseudoalteromonas,Pseudomonas,Psychrobacter,and Bromothrix,among which Pseudoalteromonas was able to adapt to high salt concentration environments and was the main pathogenic bacteria for microbial spoilage in S.crassicornis.During the middle storage period,the order of microbial species diversity is 3 kV group＞2 kV group＞control group,and electric field can effectively inhibit the growth of dominant bacteria;at the end of storage,the species structures of the three groups were similar,and there was no significant effect of electric field on the microbial community structure of shrimp meat at this time.In the PCoA principal component analysis diagram,it was found that the distances between groups G1,G2,and G3 in the mid storage period were relatively close in the coordinate system,while the distances between groups G4,G5,and G6 in the late storage period were relatively close,indicating that the microbial population structure of each group during the same storage period was similar.In summary,electric fields can inhibit the growth of dominant bacteria before and during storage,resulting in higher microbial species diversity in shrimp meat,and 3 kV is better.At the end of storage,electric fields have little impact on species diversity.
Solenocera crassicornishigh throughput sequencingmicrobial diversitylow voltage electrostatic field
万方数据
维普
