IDENTIFICATION AND ANALYSIS OF TUMOR NECROSIS FACTOR RECEPTOR-ASSOCIATED FACTORS(TRAF)AND THEIR EXPRESSION PATTERN IN SEBASTES SCHLEGELII AFTER EDWARDSIELLA PISCICIDA INFECTION
Tumor cell necrosis factor receptor-related factor(tumor necrosis factor receptor-associated factors,TRAF)is an intracellular factor with information conduction effect in the body,which is responsible for the signal transduction efforts in many receptor families and plays an important role in both innate immunity and acquired immunity.As an intracellular adaptor protein,TRAF has a certain influence on the activation of multiple signaling pathways,including cell proliferation,survival and apoptosis participate in inflammatory and immune responses.At present,studies have shown that TRAF genes in fish also play an important role.Therefore,the whole genome of TRAF gene of Sebastes schlegelii was identified based on the database of genome and transcriptome.A total of nine TRAF genes were identified in S.schlegelii,and the length and number of amino acids of these TRAF genes were analyzed.Meanwhile,the structural characteristics of these nine TRAF genes were analyzed.Six species of fish including S.schlegelii were selected for the collinearity analysis of TRAF gene.Results show that the TRAF genes has conserved collinearity in bony fish.Through phylogenetic analysis,the evolutionary relationship of TRAF family genes was better revealed,and the accuracy of identification and nomenclature of TRAF family genes was confirmed.In addition,protein-protein interaction network prediction and expression pattern of TRAF family genes were analyzed.The expression patterns of TRAF genes stimulated by Edwardsiella piscicida at different time points in the infected S.schlegelii were studied.Results show that all the TRAF genes except TRAF4.1 were up-regulated in different degrees after infection.This study revealed the structure and function of TRAF family genes at molecular level,and the immune pathway that TRAF may depend on,laying a foundation for further exploring the role of TRAF family genes in the innate immunity of S.schlegelii.
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