In this study,the hybrid grouper(Epinephelus fuscoguttatus♂×E.lanceolatus♂)and orange-spotted grouper(Epinephelus coioides),important breeds in the grouper culture industry with high economic value,were selected to determine differences in the composition and function of their intestinal microbiota using 16S rDNA high-throughput sequencing.Hybrid grouper intestinal microbiota mainly comprised Proteobacteria,Firmicutes,and Actinobacteria,whereas Proteobacteria,Fusobacteria,and Firmicutes were predominant in the orange-spotted grouper.Both breeds had a high abundance of Vibrio at the genus level in their guts.In addition,orange-spotted grouper and hybrid grouper intestinal marker bacteria included Cetobacterium,Photobacterium,and Propionigenium and Corynebacterium 1,Proteus,Staphylococcus,and Myroides,respectively.Network analysis revealed that Proteobacteria predominated the microbial network in the guts of the two grouper species.Functional prediction showed that ascorbate and aldarate metabolism,plant-pathogen interactions,and insulin signaling pathways were significantly higher in hybrid grouper gut microbiota.The intestinal microbiota of the orange-spotted grouper was significantly enriched in pathways related to vancomycin group antibiotic and streptomycin biosynthesis,linoleic acid metabolism,bisphenol degradation,and flavonoid biosynthesis.This study will deepen the understanding of grouper gut microflora and provide a reference for selecting suitable grouper culture species to improve the economic benefits of the grouper culture industry.
维普
万方数据