EFFECTS OF LYSINIBACILLUS SPHAERICUS ON GROWTH,INTESTINAL FLORA AND METABOLISM OF JUVENILE ZEBRAFISH(DANIO RERIO)
The purpose of this study was to investigate the effects of Lysinibacillus sphaericus added to cultured water on the growth,antioxidant immunity,intestinal flora and metabolism of juvenile zebrafish.270 juvenile zebrafish with an initial body weight of(0.019±0.003)g were randomly divided into 3 groups:ZF0 group(the control group,the basic diet),ZF5 group(the basic diet+105 CFU/mL L.sphaericus)and ZF7 group(the basic diet+107 CFU/mL L.sphaericus).The experiment period was 33 days.The results showed as follows:(1)There were no significant differences in the final body weight,weight gain rate and specific growth rate among all groups(P＞0.05).(2)The GPT activity of ZF5 and ZF7 groups was significantly higher than that of ZF0 group(P＜0.05),and the AKP activity of ZF5 group was significantly higher than that of other groups(P＜0.05).(3)The activities of trypsin and lipase in ZF7 group were significantly increased compared with those in ZF0 and ZF5 groups(P＜0.05).(4)Villus height and V/C in ZF7 group were significantly higher than those in ZF0 and ZF5 groups(P＜0.05);The crypt depth of ZF7 group was significantly lower than that of the other two groups(P＜0.05).(5)In terms of intestinal flora composition,ZF5 and ZF7 groups significantly decreased the abundance of Clostriobacteria and significantly increased the abundance of Actinobacteria.On the genus level,the abundance of Gordonia,Microbacterium and other beneficial bacteria significantly increased in experimental group(P＜0.05).(6)Intestinal metabolites of ZF5 and ZF7 groups were significantly different from those of ZF0 group,Lipoxin a4,Prostaglandin g2 and 11-dehydrothromboxane b2 were significantly upregulated(P＜0.05)and 5'-Phosphoribosyl-5-amino-4-imidazolecarboxamide(aicar)was significantly downregulated(P＜0.05).Differential metabolites were mainly concentrated in ABC transporters,neuroactive ligand-receptor interaction,alanine,aspartate and glutamate metabolism pathways.In conclusion,the addition of L.sphaericus in cultured water can improve the intestinal digestive performance,microbial diversity and metabolites of juvenile zebrafish.
Lysinibacillus sphaericusjuvenile zebrafishgrowth performanceantioxidant and immunityintestinal florametabolism
万方数据
维普
