MORPHOLOGAL CHARACTERISTICS AND MITOCHONDRIAL GENOME OF GYMNOTHORAX ODISHI,A NEW-RECORD GYMNOTHORAX SPECIES OF CHINA
To enrich the species information of genus Gymnothorax,we reported a new-record species of the genus Gymnothorax—Gymnothorax odishi that was collected from Lingshui County,Hainan Province,South China.This species was first discovered along the east coast of India,Bay of Bengal in 2018 and had no record in other area worldwide.Detail morphological characterizations of the new-record species are described here,and the complete mitochondrial genome were determined for molecular identification and phylogenetic construction.The distinguishable characteristics of G.odishi are:body stout,uniform brown,with small black patches behind eyes,dark rim at gill opening,and numerous small orange spots distributed from snout to the gill opening.Maxillary teeth and median intermaxillary teeth are uniserial,dentary teeth biserial,and the mean vertebral formula is 4-52-135.The complete mitogenome sequence of G.odishi was determined to be 16 580 bp in length,typically consisting 13 protein coding genes,22 tRNAs,2 ribosomal RNA genes,and 1 non-coding region.Genome organization and arrangement are identical to those of other vertebrates.The phylogenetic relationship of the Muraenidae species based on 12 protein-coding genes and Gymnothorax species based on COI genes were constructed to recover the phylogenetic status of G.odishi.In the molecular phylogenetic trees of Muraenidae species constructed from 12 protein-coding genes,G.odishi is tightly clustered with Gymnothorax reevesii and Gymnothorax pseudothyrsoideus.In the phylogenetic tree of Gymnothorax species constructed from COI gene,G.odishi is closely related to G.pseudothyrsoideus,G.reevesii,Gymnothorax hepaticus,and Gymnothorax monochrous.By the COI genetic distance analysis,the distance values between G.odishi and other Gymnothorax species range from 0.082 to 0.263,which are greater than the minimum species identification value of 0.020,revealing that G.odishi is a distinct species at both morphological and molecular level.In addition,this G.odishi is distributed in the coastal area of China and has been recognized as a new recorded species,which provided the taxonomic basis for systematic classification and species list updating of Gymnothorax species of China.
万方数据
维普
