DIVERSITY OF FUNGAL COMMUNITY IN TISSUES OF MYTILUS CORUSCUS AND ITS CULTIVATION SEAWATER
Marine fungi are widely involved in the material cycle and energy flow of offshore ecosystems.In addition,they have complex interactions with marine animals.To comprehend the correlation between marine fungi and mussel farm,seawater samples and 8 tissues of Mytilus coruscus were opted as the study's subjects.qRT-PCR and ITS rDNA high-throughput sequencing to examine the fungal abundance and structure in tissues of M.coruscus and the seawater.Results indicated that there is a significantly higher abundance of fungi within the mussel farming region and at the marginal area compared to that outside of the farming region.A total of 1 409 operational taxonomic units(OTUs)were detected from the seawater and tissues samples.Sordariomycetes was the dominant class in the seawater fungal community,while Leotiomycetes(feet:20.13％,kidneys:14.72％),Dothideomycetes(gills:2.89％,adductor muscle:1.92％,hemolymph:1.36％),Eurotiomycetes(gonads:3.59％,foot:1.57％)and Agaricomycetes(gonads:3.09％,digestive glands:2.71％,gills:2.50％)dominated the tissues.Diversity analysis illustrated significant differences in fungal communities between M.coruscus and the seawater.Bray-Curtis similarity distance analysis revealed that the mussel tissue-scaled fungal community was more similar to that in seawater within the farm and more distant from that at the marginal and outside seawater.Differences in Beta diversity between different tissues and among seawater in different regions were mainly due to species replacement;differences between mussels and seawater were mainly due to abundance variation.In summary,the fungi in M.coruscus were tissue-differentiated and the mussel disturbance have altered the fungal communities of seawater in the farming area.The results will provide a basis for mussel-associated fungal resource,interactions and ecological effects.
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