EFFECTS OF ASTAXANTHIN ON GROWTH PERFORMANCE,SERUM BIOCHEMISTRY,INTESTINAL FREE RADICAL LEVELAND INTESTINAL FLORA OF JUVENILE AMERICAN EEL(ANGUILLA ROSTRATA)UNDER DIETARY HISTAMINE STRESS
Oxidative stress is one of the major ways in which dietary histamine stress could cause detrimental effects on fish.It is important to study the alleviation effects of dietary astaxanthin with strong antioxidant capacity on the dietary histamine stress in juvenile American eels(Anguilla rostrata).Four hundred trial fish in initial body weight of(25.01±0.17)g/fish were randomly divided into four treatment groups.They were the control group fed a basal diet,the H group fed a diet with a high level of histamine(662 mg/kg),the H+Ast4 group fed the high histamine diet with 4 mg/kg astaxanthin,and the H+Ast8 group fed the high histamine diet with 8 mg/kg astaxanthin.Compared with the control group,the growth performance and non-specific immunity in the serum of juvenile A.rostrata in the H group were significantly decreased(P＜0.05),the levels of intestinal free radicals in the H group were significantly increased(P＜0.05),and the relative abundances of potentially pathogenic bacteria were increased.The growth performance and non-specific immunity in the serum of juvenile A.rostrata in the H+Ast groups were significantly higher than those in the H group(P＜0.05),and the levels of intestinal free radicals were significantly lower than those in the H group(P＜0.05),no significant difference in the aforesaid parameters was found between H+Ast groups and the control group(P＞0.05).The relative abundances of the potential probiotics in the H+Ast groups were higher than those in the H group and control group.In conclusion,the addition of 4 mg/kg astaxanthin could alleviate the growth retardation of juvenile A.rostrata induced by dietary histamine stress,improve serum non-specific immunity,reduce the level of intestinal free radicals,and regulate intestinal microflora beneficially.Dietary 8 mg/kg astaxanthin might not exert further improvement in all parameters of juvenile A.rostrata in the present trial.These results provide certain references for astaxanthin alleviating dietary histamine stress and promote its application in the feed for A.rostrata.
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