STUDY ON BEHAVIORAL RESPONSE OF ZEBRAFISH(DANIO RERIO)UNDER CADMIUM STRESS
Online biomonitoring techniques can effectively assess water quality changes.An online monitoring system of fish behavior was developed in laboratory,based on which the behavioral response of zebrafish(Danio rerio)in 15 d under 0.001 mg/L and 0.01 mg/L cadmium stress was studied,and the experimental data were processed for differential analysis,autocorrelation analysis(autocorrelation)and self-organized neural networks(SOM)analysis.Results show that the mean behavioral intensity of the control zebrafish was 0.68±0.14,those of 0.001 mg/L group and 0.01 mg/L group were 0.65±0.15,and 0.56±0.13,respectively,which showed that cadmium stress caused behavioral inhibition of zebrafish,and the degree of inhibition increased significantly with the increase of the cadmium concentration(P＜0.001).In addition,the intensity of the behavior in photoperiod in the control group(0.75±0.14)was significantly higher than that in dark period(0.61±0.13;P＜0.001).The behavioral intensities in photoperiodic of 0.001 mg/L and 0.01 mg/L groups(0.69±0.15 and 0.60±0.13,respectively)were higher than those in dark period(0.56±0.16 and 0.48±0.13;P＜0.001,respectively),indicating that the zebrafish is circadian rhythmic species.Moreover,the behavioral response showed a distinct 24-h rhythm revealed by SOM analysis.Cadmium stress could inhibit zebrafish behavior in positive relationship with the cadmium concentration;zebrafish behavior response had a 24-h rhythm,which was greater in photoperiod than that in dark period.This study analyzed the behavioral response rules of zebrafish under cadmium stress through the fish behavior online monitoring system,so as to achieve the goal of online monitoring water quality and assessing pollution status,provided a data support to a biological online monitoring system on water quality control and pollution assessment.
万方数据
维普
