EXCAVATION OF HAEMATOCOCCUS PLUVIALIS STRAINS WITH EXCELLENT TRAITS BASED ON THE PHOTOSYNTHETIC MUTATION POOL
Haematococcus pluvialis Flotow is the main source of natural astaxanthin.However,the high-cost,low-efficiency large-scale production of astaxanthin are hard to meet the market demand.Establishing a screening strategy to quickly obtain high-quality mutant strains will help improve the efficiency of germplasm resource development of H.pluvialis and obtain high-quality algal strains in a high astaxanthin enrichment.Haematococcus pluvialis NIES-144 was used as the starting algal strain,and a photosynthetic mutant pool was obtained through EMS mutagenesis at a high-light stress.Using chlorophyll fluorescence parameters as detection indicators,mutant algal strains with changes in growth rate and astaxanthin accumulation ability were screened quickly.Eleven algae strains were randomly selected from the photosynthetic mutant pool for testing.Results show that the photosynthetic mutant strains were different from the mock strain in terms of proliferation ability and astaxanthin accumulation ability.Forty-five percent of the mutant strains were significantly better than the mock strain in proliferation ability and astaxanthin accumulation ability.The photochemical activity was associated with the proliferation of H.pluvialis and its astaxanthin accumulation ability.Therefore,the strategy of mining H.pluvialis with excellent traits based on photosynthetic mutation pool is feasible,which provided a new insight on acquisition of excellent microalgae strains.