ANALYSIS OF PIGMENT CELL CHANGES AND CONTROL GENES EXPRESSION IN THE DEVELOPMENT OF BODY COLOR PATTERN OF AMPHIPRION OCELLARIS:Ⅱ.LARVAE,JUVENILE AND YOUNG FISH
This study recorded the developmental process of the body color pattern formation of Amphiprion ocellaris during larvae,juvenile and young periods.By comparing the characteristics of body color changes during different developmental stages,nine developmental stages with significant changes in body color patterns during the larval,juvenile and young stages were selected.Then by fluorescent quantitative PCR,the expression of 10 body color control genes during different developmental stages in fish were analyzed.The results showed that there was a clear temporal pattern in the occurrence of body color in A.ocellaris.During the larval period,the fish body appeared semitransparent,with melanocytes arranged on both sides of the body,gradually increasing in number as it grows and develops.During the juvenile period,erythrophore and xanthophore began to appear on the body surface,gradually becoming opaque;at 9 dph,the first stripe appeared,and the number of iridocyste gradually increased;at 10 dph,a second stripe was observed.During the young period,three white stripes were completely formed,and the orange red and white stripes on the body surface were separated by melanocytes,the boundaries gradually became clear,and grew into complete patterns.Based on the analysis of fluorescence quantitative PCR results,it was found that 10 color-related genes were expressed at each developmental stage in larvae,juvenile and young periods,and the expression trend of genes with different functional classifications varied greatly at different developmental stages,and the genes with large changes in expression in the early stages were mainly TYR,Dct,Ednrb,Sox 10 genes and other genes related to melanocyte migration,differentiation and synthesis.With the continuous growth and development of juveniles,white stripes appeared one by one,the expression levels of iridochromine-related Fms genes and Foxd3 genes also began to increase significantly.
Amphiprion ocellarisdevelopmentbody color patterntemporal occurrencepigment cellsexpression analysis
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