EFFECTS OF STARVATION ON THE GROWTH AND METABOLISM OF LARGE YELLOW CROAKER(LARIMICHTHYS CROCEA)AS REVEALED BY TRANSCRIPTOMIC ANALYSES
Being the most productive and extensively farmed marine species in China,prolonged and unjustified starvation can drastically reduce the aquaculture efficiency of large yellow croakers(Larimichthys crocea).Hence,it is imperative to regulate the duration of winter fasting in a reasonable manner.An experiment was conducted during winter to explore the effects of fasting on large yellow croaker.The aquaculture study took place at temperatures ranging from 9.0 to 14.3 ℃ for durations of 0,8,and 16 weeks.This investigation scrutinized the impacts of low temperature and starvation on the growth,body composition,antioxidative enzyme activity and gene expression of large yellow croaker.Findings indicated that the hepato-somatic index(HSI),viscera-somatic index(VSI),crude fat,crude protein in muscles and whole fish,and liver glycogen content all significantly decreased(P＜0.05)with prolonged starvation,whereas muscle glycogen content remained relatively unchanged(P＞0.05).The liver antioxidative enzyme malondialdehyde(MDA)exhibited an initial increase followed by a decrease;the activities of glutathione peroxidase(GSH-Px)and catalase(CAT)noticeably decreased(P＜0.05),while triglyceride(TG)activity significantly increased(P＜0.05).The transcriptome analysis results showed that the differentially expressed genes were significantly down-regulated after starvation compared to the control group.GO analysis revealed that the differentially expressed genes after starvation were mainly enriched in processes such as lipid metabolism,fatty acid biosynthesis,and complement activation.Cluster analysis indicated that after 16 weeks of starvation,the relative expression levels of carnitine palmitoyltransferase 1(CPT1),3-ketoacyl-CoAthiolase(HADHB),and carnitine palmitoyltransferase 2(CPT2)genes in the fatty acid metabolism pathway were increased,while the relative expression levels of genes for fatty acid elongase(HACD),fatty acid synthase(FASN),and 5-aminolevulinate synthase(ALAS)were decreased.The relative expression levels of genes in the immune metabolism pathway,including serpin peptidase inhibitor(SERP),fibrinogen-1(FGG-1),fibrinogen-3(FGG-3),and fibrinogen beta(FGB)were also reduced as confirmed by qRT-PCR.Therefore,during the wintering period,prolonged starvation could inhibit the growth and expression of immune-related genes,and promote the liver's antioxidant capacity and the expression of genes related to lipid metabolism.
Larimichthys croceacold and starvation stressgrowth performancebody compositionliver antioxidative capacitygene expression
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维普
