MECHANISM OF MODIFIED CLAY ON EFFECTIVE INHIBITIION AND REMOVAL OF ULVA PROLIFERA GAMETES
Micropropagules are regarded as the"seed"of green tide.Using modified clay to remove micropropagules is one of the potential methods for early control of green tide.Taking Ulva gametes as the experimental object,modified clay was tested,and the mechanism of modified clay was studied in terms of flocculation,physiology,and biochemistry.Results show the residual gametes were inhibited and removed effectively by the MC Ⅲ clay(that modified with hexadecyl trimethyl ammonium bromide).The removal efficiency of gametes under 0.05 g/L concentration of MC Ⅲreached 99.4％,and the germination rate of residual gametes was lower than 5％.Besides,the inhibition and removal efficiency were increased with the increase in MC Ⅲ concentration.Furthermore,analysis on the mechanism showed that MC Ⅲ could effectively remove gametes by destroying the cell membrane of gametes,and then enhance the removal effect by electroneutralization.The oxidative stress due to MC Ⅲ presence inhibited the physiological activity of the residual gametes,and thus slowed down the division and differentiation of the fixed gametes,resulting in slow growth and poor development of the germinated seedlings.Therefore,MC Ⅲ can effectively remove and inhibit gametes.This study provided a scientific basis for early control and effective elimination of green tide.
万方数据
维普
