首页|短凯伦藻高效溶藻菌Pseudoalteromonas shioyasakiensis SE3的分离、鉴定及溶藻作用研究

短凯伦藻高效溶藻菌Pseudoalteromonas shioyasakiensis SE3的分离、鉴定及溶藻作用研究

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微生物防控法以其生态调控功能和环境友好特征成为赤潮治理领域备受关注的一种方法.从短凯伦藻(Karenia brevis)藻际微生物中筛选出一株高效溶藻菌,研究了其对短凯伦藻的溶藻特性、溶藻作用方式及机制.经16S rDNA鉴定,该溶藻菌属于假交替单胞菌属细菌Pseudoalteromonas shioyasakiensis SE3.结果表明,菌株SE3的溶藻效果不受细菌生长阶段的影响,主要受细菌数量的影响,当细菌密度为1.2×107 cells/mL时,在6h即能达到100%的溶藻率.基于扫描与透射电镜等研究发现,菌株SE3主要以与藻细胞直接接触的作用方式溶藻,菌株SE3首先破坏短凯伦藻膜结构,侵入藻细胞内部,导致藻细胞的死亡.上述研究结果为利用藻际细菌防控赤潮提供了重要参考.
ISOLATION AND IDENTIFICATION OF AN ALGICIDAL BACTERIUM PSEUDOALTEROMONAS SHIOYASAKIENSIS SE3 AND ITS ALGICIDAL EFFECTS ON KARENIA BREVIS
In the field of red tide control,microbial methods have become an ideal approach due to its ecological regulation function and environment-friendly characteristics.A highly efficient algicidal bacterium as the algicidal mode was selected for Karenia brevis.Its algicidal characteristics,mode,and mechanism against K.brevis were studied.The algicidal bacterium was identified as Pseudoalteromonas shioyasakiensis SE3 according to 16S rDNA.Results show that the algicidal activity showed no relation to the growth stages,but the amount of bacteria.When the bacterial density was 1.2×107 cells/mL,the algicidal rate achieved 100%in 6 h.Based on results of SEM and TEM observations,the strain SE3 killed K.brevis directly.The stain SE3 destroyed the cytomembrane of K.brevis first,then invaded the interior of algal cells,and finally led to the death of cells.This study provided an important reference for preventing and controlling red tide with algal bacteria.

red tide controlmicrobial methodsalgicidal bacteriaPseudoalteromonasalgicidal mechanism

姚玉芳、吴在兴、袁涌铨、贺立燕、曹西华、宋秀贤、俞志明

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中国科学院海洋研究所 海洋生态与环境科学重点实验室 山东青岛 266071

青岛海洋科技中心海洋生态与环境科学功能实验室 山东青岛 266237

中国科学院大学 北京 100049

赤潮治理 微生物方法 溶藻细菌 假交替单胞菌 溶藻机制

青岛海洋科学与技术试点国家实验室"十四五"重大项目国家重点研发计划政府间合作项目国家自然科学基金项目

LSKJ202203700号2022YFE0136400号42306150号

2024

海洋与湖沼
中国海洋湖沼学会 中国科学院海洋研究所

海洋与湖沼

CSTPCD北大核心
影响因子:0.737
ISSN:0029-814X
年,卷(期):2024.55(4)
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