ISOLATION AND IDENTIFICATION OF AN ALGICIDAL BACTERIUM PSEUDOALTEROMONAS SHIOYASAKIENSIS SE3 AND ITS ALGICIDAL EFFECTS ON KARENIA BREVIS
In the field of red tide control,microbial methods have become an ideal approach due to its ecological regulation function and environment-friendly characteristics.A highly efficient algicidal bacterium as the algicidal mode was selected for Karenia brevis.Its algicidal characteristics,mode,and mechanism against K.brevis were studied.The algicidal bacterium was identified as Pseudoalteromonas shioyasakiensis SE3 according to 16S rDNA.Results show that the algicidal activity showed no relation to the growth stages,but the amount of bacteria.When the bacterial density was 1.2×107 cells/mL,the algicidal rate achieved 100%in 6 h.Based on results of SEM and TEM observations,the strain SE3 killed K.brevis directly.The stain SE3 destroyed the cytomembrane of K.brevis first,then invaded the interior of algal cells,and finally led to the death of cells.This study provided an important reference for preventing and controlling red tide with algal bacteria.
red tide controlmicrobial methodsalgicidal bacteriaPseudoalteromonasalgicidal mechanism