COMMUNITY STRUCTURE OF CULTURABLE HALOPHILIC AND HALOTOLERANT MICROORGANISMS IN GASIKULE SALT LAKE ON THE QINGHAI-TIBET PLATEAU AND COMPARISON OF DIFFERENT ISOLATION METHODS
Halophilic and halotolerant microorganisms have important application potential in many fields such as biomedicine and biological enzymes.To make better use of the microbial resources of salt lakes on the Qinghai-Tibet Plateau,the culture-free community composition of microorganisms in Gasikule Salt Lake was analyzed with the help of the Illumina MiSeq sequencing platform;and the methods of oligotrophic culture,enrichment culture,and gradient dilution were used to isolate and culture halophilic and halotolerant microorganisms.The culture-free high-throughput sequencing results showed that there were 30 phyla,50 classes,81 orders,162 families,and 417 genera of bacteria;and 6 phyla,7 classes,9 orders,15 families,and 39 genera of Archaea.The dominant genera of bacteria were Fodinibius and Gracilimonas,and the dominant genus of Archaea was Halorubrum.A total of 502 halophilic and halotolerant microorganisms were obtained by pure culture,including 472 strains of bacteria distributed in 4 phyla,5 classes,7 orders,9 families,and 13 genera.The dominant genera were Bacillus and Halomonas.The culturable bacteria of the genus Fodinibius of the phylum Balneolota were isolated from salt lakes for the first time.Thirty strains of halophilic Archaea were obtained by pure culture,distributed in 1 phylum,1 class,1 order,3 families,and 5 genera;and the dominant genus was Halorubrum.Comparing the isolation results under different culture conditions,we found that the 1/2 TSA medium and 1/2 RCA medium were more conducive to the isolation of halophilic and halotolerant bacteria,and the optimal enrichment duration were 7 days and 30 days,respectively.Dilution gradients of 10-1 and 10-2 could complement the undiluted samples to increase the diversity of culturable halophilic and halotolerant bacteria.The 1/10 2216E medium was more suitable for the isolation of Archaea,the optimal enrichment days were 7 and 14,respectively,and the samples were better separated without dilution.This study provided a theoretical basis and data support for the optimization of culturable microorganism isolation scheme in salt lakes on the Qinghai-Tibet Plateau.
Gasikule Salt Lakehalophilic and halotolerant microorganismsisolation culturebiodiversityhypersaline environment
万方数据
维普
