CHARACTERIZATION OF PHYSIOLOGICAL,BIOCHEMICAL,AND HISTOLOGICAL RESPONSES OF SEPIELLA JAPONICA UNDER SALINITY STRESS
Salinity is a key ecological factor affecting the metabolism and osmoregulation of marine organisms.Changes in salinity can affect energy expenditure,embryonic development,individual growth,immunity,reproduction,serum osmolality,ionic concentration,antioxidant capacity,and free amino acid content in cephalopods.To investigate the effects of salinity stress on Sepiella japonica,adult cuttlefish were subjected to high(32)and low salinity(18)conditions.Results showed that the concentrations of Na+,Cl-,K+,and Ca2+in the hemolymph of the cuttlefish decreased significantly under 6,12,and 24 hours of low salinity stress exposure.Additionally,the Na+concentration in the hemolymph increased significantly after 12 and 24 hours of high salinity stress treatment,while the Ca2+concentration increased significantly after 12 hours treatment.A significant decrease in SOD activity and CAT expression was observed in the liver after 6 hours salinity stress exposure.After 24 hours low salinity stress treatment,the mRNA expression level of Na+/K+-ATPase a-subunit and Ca2+-ATPase in the gills significantly decreased.Moreover,the H&E staining revealed a decrease in the nucleocytoplasmic ratios of the cuttlefish gill and kidney tissues,and pathological damage to the liver,which remained unchanged after high salinity stress exposure.The findings provide a morphological support and a theoretical basis for studying the salinity response of S.japonica and cephalopods,and further have practical implications for dealing with salinity mutations in cuttlefish culture.
Sepiella japonicasalinity stressphysiological and biochemical characteristicsenzyme activityhistologic analysis
万方数据
维普
