METAMORPHOSIS OF MIZUHOPECTEN YESSOENSIS LARVAE INDUCED BY NAVICULA SP.AND ITS PROTEOMIC RESPONSE
To understand the mechanism of the effect of Navicula sp.film substract on the settlement and metamorphosis of Mizuhopecten yessoensis larvae,the settlement and metamorphosis rates of the larvae and the proteomic differences were investigated under four concentrations of Navicula sp.solution(1A,10A,100A,and 1 000A;A is the multiple of dilution of the original concentration)and sterilized natural seawater as the control.Results show that the highest metamorphosis rate(45％)was found in Group 10A(1 366/mm2),which differed significantly(P＜0.01)from that of the control(13％).Too high or too low concentration resulted in a lower induction rate.Using non-labeled quantitative techniques(4D label free)proteomic analysis to compare between Navicula sp.filmed substract and the control group larvae,we found that 316 proteins were significantly up-(198)or down-regulated(118)after induction by the Navicula sp.biofilm.The expressions of cathepsin K,cathepsin L,cartilage matrix proteins,collagen,ferritin,glutathione S-transferase,glutathione peroxidase,and lectin were elevated,while those of heat shock proteins 70 and NAD(P)H oxidase(forming H2O2)proteins were decreased.Proteins such as histone K,histone L,and heat shock proteins were associated with apoptosis,indicating that there is substantial apoptosis during larval settlement and metamorphosis.Proteins such as cartilage matrix proteins,collagen,and ferritin were associated with calcium carbonate shell formation,indicating that larvae induced by Navicula sp.film were in the process of completing the construction of adult shells.Proteins of glutathione S-transferase,glutathione peroxidase,and NAD(P)H oxidase(forming H2O2)were associated with immune function,indicating the maintenance of cellular homeostasis during larval settlement and metamorphosis.C-type lectins and galectin were bound to polysaccharides to trigger larval settlement and metamorphosis.The up-and down-regulation of these differential proteins were closely related to morphological changes in larvae during settlement and metamorphosis.The effective component of the biofilm inducing the settlement and metamorphosis of the scallop larvae might be the extracellular polysaccharides produced by Navicula sp.After detecting the polysaccharides on the surface of the Navicula sp.filmed substract,the lectins in the larvae would bind specifically to the polysaccharides,inducing the larvae to complete settlement and metamorphosis.This study provided a theoretical basis for artificial cultivation of bivalve seedlings.
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维普
