THE SUCCESSIONAL DIFFERENCE IN STRUCTURE AND COMPOSITION OF COMMENSAL BACTERIAL COMMUNITIES IN THE LARVAE OF CRASSOSTREA SIKAMEA,C.ANGULATA,AND C.GIGAS WITH HOST DEVELOPMENT
To understand whether there exist differences in the succession of commensal bacterial communities in the larvae of Crassostrea sikamea,C.angulata,and C.gigas with host development,the structure and composition of commensal bacterial communities among the three oyster larvae at four developmental stages were compared via 16S rRNA high-throughput sequencing technology.Results showed that a total of 3 484 bacterial OTUs were identified in the four developmental stages of the three oyster larvae,among which the numbers of shared OTUs in the D-shaped,veliger,pediveliger,and spat stages accounted for 43.1％,24.9％,31.3％,and 36.8％of total OTUs,respectively.The dominant bacteria at four developmental stages were attached to Proteobacteria,Bacteroidetes,and Actinobacteria,and their abundance varied in different larval developmental stages.In particular,significant differences were observed at the bacterial genus level.The diversity of commensal bacterial communities of the three oyster larvae was different in four developmental stages.The bacterial diversity was the highest in C.gigas larvae at pediveliger stage,but was the lowest in C.sikamea larvae at veliger stage.Notably,the structure of commensal bacterial communities among the three oyster larvae showed similar succession change with host development,and the significant variation was found at same development stage.Furthermore,comparison of the potential functions of its commensal bacterial communities at different developmental stages showed that the abundances of functional pathways involved in the carbohydrate biosynthesis and metabolism,lipid metabolism,and immune system were the highest at the D-shaped stage,whereas those involved in the amino acid metabolism,cell growth and death,and biosynthesis of other secondary metabolites were the highest at the spat stage but the lowest at veliger stage.These results indicate that there were significant differences in the structure and composition of commensal bacterial communities in the larvae of C.sikamea,C.angulata,and C.gigas at the four developmental stages,which may be influenced by host genetics.
oyster larvaecommensal bacterial communitydevelopment stagestructural and compositional characteristicssuccessive change
万方数据
维普
