The frequent occurrence of cyanobacterial blooms has constrained the development of Chinese mitten crab(Eriocheir sinensis)culture,and the mechanism of microbial community response to cyanobacterial blooms is an important part of the prevention and management of cyanobacterial blooms.To understand the characteristics of the bacterial community response to the cyanobacterial blooms in the mitten crab culturing system,the 16S rRNA gene amplicon sequencing was used to investigate the changes of the bacterial community.Results show that the dominant bacterial taxa in the water were Burkholderiaceae and Microbacteriaaceae,and the dominant taxa in the sediment were Steroidobacteraceae and Burkholderiaceae.At the family level,the appearance of cyanobacteria was significantly positively correlated with most other bacterial communities.Potential pathogens in the water showed an opposite trend to the intensity of cyanobacterial blooms,while in the sediment the potential pathogens were enriched and characterized by mainly OTU1280(Escherichia coli).CCA results showed that temperature,NO3--N,NO2--N,and NH4+-N drove the variation in bacterial taxa of the cyanobacteria in the water during cyanobacterial blooms,and that NO2--N was the dominant factor driving the variation of the potential pathogens in the water.In addition,the succession pattern of bacterial communities and the enrichment characteristics of potential pathogens in the mitten crab pond culturing system under cyanobacterial blooms were preliminarily investigated.This study provided a valuable data support for the subsequent management of cyanobacterial blooms in mitten crab culturing pond.
关键词
中华绒螯蟹/池塘养殖系统/水华/16S/rRNA基因扩增测序/细菌群落/潜在致病菌
Key words
Chinese mitten crab/pond culturing system/cyanobacterial bloom/16S rRNA gene amplicon sequencing/bacterial community/potential pathogens
维普
万方数据