GENETIC DIVERSITY OF FOUR MUDSKIPPER(GOBIIDAE)IN THE NORTHWEST PACIFIC BASED ON MTDNA ND5 GENES
Mudskippers are a collective term for amphibious fish widely distributed in the Northwest Pacific,possessing significant economic and ecological value.A comparative analysis was conducted on the population genetic diversity and genetic structure of four mudskipper species in the Northwest Pacific,namely,Periophthalmus modestus,Periophthalmus magnuspinnatus,Boleophthalmus pectinirostris,and Boleophthalmus boddarti,based on the mitochondrial ND5 gene sequence.Results show that,in the 549 bp mitochondrial ND5 sequence,the haplotype diversity of P.modestus was the highest,followed by B.boddarti,B.pectinirostris,and P.magnuspinnatus.The nucleotide diversity of B.pectinirostris was the highest,followed by P.modestus,B.boddarti,and P.magnuspinnatus.The constructed neighbor-joining tree based on haplotypes showed that the above four mudskipper species were clustered into separate clades,but there was no significant phylogenetic structure within each species.The Fst(fixation index,a measure of population differentiation in genetic structure)results of P.modestus revealed significant differentiation between the Chinese and Japanese populations.For B.pectinirostris,the Fst values among populations Zhanjiang,Xiamen,and Wenzhou were proportional to geographical distance.In addition,as AMOVA(analysis of molecular variance)indicate,the molecular variation came mainly from within populations,indicating a relatively high level of differentiation within populations.Meanwhile,the neutral tests,nucleotide mismatch distribution,and Bayesian skyline plot analysis all suggested that mudskipper and B.pectinirostris might have experienced a population expansion event at the end of the Pleistocene.This study provided a comprehensively comparative analysis of genetic variation of four mudskipper species in the Northwest Pacific,and offered fundamental data for their resource conservation and rational utilization.
万方数据
维普
