EFFECTS OF INORGANIC NITROGEN AND HEAVY METALS ON ANAEROBIC AMMOXIDATION BACTERIA IN SEDIMENTS IN DIFFERENT REGIONS OF BOHAI SEA
Anaerobic ammonium oxidation(anammox)is an essential process for removing nitrogen from marine sediments.However,the effects of inorganic nitrogen and heavy metals on anaerobic ammonium oxidation remain unclear.To assess their community structure and the impact of heavy metal content on diversity,the anaerobic ammonium-oxidizing bacteria in the sediments from 13 sampling sites in the Bohai Sea,North China was analyzed.Illumina high-throughput sequencing technology and real-time fluorescence quantitative PCR technology were employed to detect the AMX16S rDNA gene in the surface sediments.Results show that that anaerobic ammonium-oxidizing bacteria in the sediments of the Bohai Sea belonged to 14 phyla,8 classes,9 orders,5 families,8 genera,and 26 species.Planctomycetota was the predominant species in the Bohai Sea sediments,and particularly abundant in the central region.The genus Candidatus Scalindua exhibited the highest relative abundance.Heavy metal Hg concentration in sediments was highest in the southern region.Random forest analysis showed that the structural changes of the anaerobic ammonia-oxidizing bacteria community in the southern,northern,and central region may be closely related to the NH4+,Hg,Cr contents;Pb content;and NO2 content,respectively.AMX16S rDNA abundance positively correlated with Cr,Cu,TN,and sediment grain size(P＜0.05).The results demonstrate abundant microbial resources and anaerobic ammonium-oxidizing bacteria in the Bohai Sea,which possess a potential to metabolize various pollutants.These findings lay the groundwork for comprehending the geographical distribution patterns and community niche differentiation of microorganisms influenced by offshore pollution.
Bohai Sea sedimentanaerobic ammoxidation bacteriaenvironmental factorsheavy metal
万方数据
维普
