MICROEUKARYOTE DIVERSITY AND THEIR CORRELATIONS WITH BACTERIA IN THE SHALLOW SEAMOUNT AREA OF THE WESTERN PACIFIC OCEAN BASED ON THE ENVIRONMENTAL RNA
Seamounts are distributed throughout the ocean floor.Due to their special topography and hydrodynamic environment,seamounts are inhabited by diverse and unique biological communities.In this study,the diversity and distribution of microeukaryotes in the M4 seamount area of the Western Pacific Ocean were investigated by the environmental RNA technology,and then the results were compared with those obtained by the environmental DNA technology.We found that the community structures at the surface and deep chlorophyll maximum layers detected by RNA and DNA techniques were similar,but significantly different in water layers deeper than 200 m.The relative abundance of Syndiniales in RNA data was lower than that in DNA data,suggesting a possible overestimation on Syndiniales.On the contrary,the relative abundance of Dinophyceae and Chrysophyceae may be underestimated by the eDNA.We further revealed that Dinophyceae were a key group in the co-occurrence network of the DCM layer,but their interaction with bacteria was weak and their metabolic activity was low.Mixotrophic groups of Chrysophyceae and Bicoecea were commonly found in deep sea water with higher metabolic activity.Environmental RNA technology,which can detect the distribution of species with life activity,can provide a more comprehensive understanding of the diversity and distribution of microeukaryotes in seamount areas.
deep seaenvironmental RNAampliconprotist diversityseamount effect
万方数据
维普
