GUT MICROORGANISMS DIFFERENCES AND THEIR EFFECTS ON ENVIRONMENTAL ADAPTABILITY OF ARTEMIA IN TWO DIFFERENT HABITATS
Artemia,a small crustacean living in high salt water,can tolerate a variety of extreme environments and exhibit different physiological adaptations in different habitats.However,little is known about the composition of gut microbiota in Artemia from different altitudes and their adaptive mechanisms to the environment.In this study,16S rRNA high-throughput sequencing and metabolomics were used to analyze the gut microbiota of Artemia from Lagkor Co(A.tibetiana)at high altitude and Artemia from Bohai Bay(A.franscicana coexist with A.parthenongenetica)at low altitude.The results showed a significant increase in the abundance of Proteobacteria,Chloroflexi,and Vibrio in the gut of Artemia from Lagkor Co,while Halolactibacillus,Roseovarius,and other genera were more dominant in the gut of Artemia from Bohai Bay.Metabolomic analysis showed that compared with Artemia from low altitudes in Bohai Bay,Artemia from Lagkor Co exhibited significant enrichment in metabolic pathways related to antibiotic biosynthesis and unsaturated fatty acid biosynthesis.There were significant accumulation of pentalenolactone,roseflavin,and essential fatty acids for fatty acid synthesis including eicosapentaenoic acid(EPA),alpha-linolenic acid,and fatty acyl-CoA(P＜0.05).The accumulation of these metabolites may help enhance the ability of A.tibetiana to adapt to the harsh environment at high altitude.These results revealed that there were significant differences in gut microbiota composition and metabolic characteristics between Artemia at high and low altitudes,the two habitats at high and low altitudes,which provided important insights into environmental adaptation mechanisms of Artemia in different environments.
万方数据
维普
