SCREENING AND OPTIMIZATION OF EFFICIENT HETEROTROPHIC AMMONIA OXIDIZING BACTERIA AND APPLICATION
In order to achieve efficient and rapid degradation of ammonia nitrogen in aquaculture water or tailwater,the screening and optimization of ammonia-oxidizing bacteria and its application in aquaculture tailwater treatment were studied.A strain of ammonia-oxidizing bacteria NH-1 with heterotrophic nitrification was screened from pond active sludge.Strain NH-1 was identified as Pseudomonas sp.by 16S rDNA molecular biology.Strain NH-1 was sensitive to most antibiotics and was a y-hemolytic strain that did not produce hemolysin.Under laboratory conditions with glucose as the carbon source,water temperature of 24.9 ℃,pH 8.1,C/N 9.1,and inoculation volume of 0.7％,the degradation rate of ammonia nitrogen in aquaculture tailwater was 96.3％after 24 h of hydraulic retention time.In the application experiment of industrial eel farming tailwater treatment,the degradation rate of ammonia nitrogen was 95.9％after 24 h of hydraulic retention time.The results showed that strain NH-1 could degrade ammonia nitrogen in aquaculture tailwater safely and efficiently,and had good application prospects.
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