In order to achieve efficient and rapid degradation of ammonia nitrogen in aquaculture water or tailwater,the screening and optimization of ammonia-oxidizing bacteria and its application in aquaculture tailwater treatment were studied.A strain of ammonia-oxidizing bacteria NH-1 with heterotrophic nitrification was screened from pond active sludge.Strain NH-1 was identified as Pseudomonas sp.by 16S rDNA molecular biology.Strain NH-1 was sensitive to most antibiotics and was a y-hemolytic strain that did not produce hemolysin.Under laboratory conditions with glucose as the carbon source,water temperature of 24.9 ℃,pH 8.1,C/N 9.1,and inoculation volume of 0.7％,the degradation rate of ammonia nitrogen in aquaculture tailwater was 96.3％after 24 h of hydraulic retention time.In the application experiment of industrial eel farming tailwater treatment,the degradation rate of ammonia nitrogen was 95.9％after 24 h of hydraulic retention time.The results showed that strain NH-1 could degrade ammonia nitrogen in aquaculture tailwater safely and efficiently,and had good application prospects.
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