Gracilariopsis lemaneiformis is the second largest mass culture seaweed in China.In this study,we used G.lemaneiformis as the material and investigated the effects of five different concentrations of 1-aminocyclopropane-1-carboxylic acid(ACC)on the carpospore release of G.lemaneiformis.It was observed that 250 μmol/L of ACC showed the most optimal effect in promoting spore release.Following the exogenous addition of 250 μmol/L ACC,the photosynthesis of G.lemaneiformis further declined on the second and fifth days.Additionally,spore release in G.lemaneiformis was closely associated with the antioxidant system.By the fifth day after ACC treatment,the activities of superoxide dismutase(SOD),peroxidase(POD),and glutathione reductase(GR),as well as the content of reduced ascorbic acid(AsA),significantly increased.Moreover,transcriptomic analysis revealed that on the fifth day after ACC treatment,the expression levels of genes associated with the photosynthetic pathway were downregulated.Conversely,genes related to glycolysis,tricarboxylic acid(TCA)cycle,and nitrogen metabolism pathways were significantly upregulated.Finally,exogenous ACC significantly upregulated genes related to jasmonic acid synthesis pathway,while showing no significant effect on genes related to the ethylene synthesis pathway.This study demonstrated the role of ACC in promoting the release of carpospores in G.lemaneiformis,providing new techniques for the germplasm breeding of G.lemaneiformis.
维普
万方数据