首页|金虎杂交斑三倍体、二倍体生长、发育比较及形态性状差异分析

金虎杂交斑三倍体、二倍体生长、发育比较及形态性状差异分析

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为了解金虎杂交斑三倍体的生长与形态特征,利用金虎杂交斑三倍体(JH-3N)和二倍体群体(JH-2N),通过对比养殖试验分析了两种不同倍性杂交石斑鱼的生长性能,同时采用多元统计分析方法对JH-3N、JH-2N及母本棕点石斑鱼(EF)和父本蓝身大斑石斑鱼(ET)的形态特征进行统计分析.结果显示,经90 d对比养殖后,JH-3N的终末体质量较JH-2N提高9.59%.性腺组织学观察表明,JH-3N卵巢与同期JH-2N相比发育明显滞后.经卡方检验显示,JH-3N与JH-2N、父本和母本的7个可数性状都没有显著性差异.单因素方差分析显示,4个石斑鱼群体除M14(臀鳍前端基部至尾鳍长)外,其他18个比例性状差异显著(P<0.05).聚类分析显示,JH-3N为单独一支,与其他3个群体分离.主成分分析共得到累计贡献率达63.273%的3个主成分,差异主要体现在鱼体的背腹轴方向.判别分析显示,4个石斑鱼群体的判别准确率达100%,综合判别率为100%.研究结果表明,JH-3N具有生长速度更快、性腺发育滞后的优良性状,表型性状与JH-2N相比具有显著性差异,具备在生产上推广应用的前景,研究结果为金虎杂交斑倍性育种及种群鉴定提供了丰富的生物学基础数据.
COMPARISON ANALYSIS OF GROWTH,DEVELOPMENT,AND MORPHOLOGICAL CHARACTERISTICS TRIPLOID AND DIPLOID HYBRID GROUPERS
The objective of this study was to gain insight into the growth and morphological characteristics of triploid hybrid groupers.The growth performance of these two different ploidy hybrid grouper populations was analyzed through a comparison culture using hybrid grouper triploids(JH-3N)and diploid populations(JH-2N),while multivariate statistical analysis was also employed to statistically analyze the morphological characteristics of JH-3N,JH-2N and the maternal Epinephelus fuscoguttatus(EF)and paternal Epinephelus tukula(ET).The results showed that the terminal body weight of the JH-3N was 9.59%higher than that of the JH-2N after the 90-day of comparative culture.Histological observation of the gonads showed that the development of the JH-3N ovaries was significantly delayed compared to that of the JH-2N during the same period.The Chi-square test demonstrated that none of the seven countable traits exhibited significant differences between the JH-3N and JH-2N populations and their parents.The one-way ANOVA demonstrated that the four grouper groups exhibited statistically significant differences(P<0.05)in 18 proportional traits,with the exception of M14(anterior base of anal fin to caudal fin length).The results of the cluster analysis indicated that the JH-3N group was a discrete entity,distinct from the other three groups.Principal component analysis yielded a total of three principal components,which collectively contributed 63.273%to the total variance,which mainly reflected the dorsoventral axis morphological variables of the fish.The results of the discriminant analysis demonstrated that the four grouper groups exhibited 100%discrimination accuracy,and the comprehensive discrimination rate was also 100%.The results demonstrated that JH-3N exhibited superior growth rates and delayed gonad development,while displaying distinct phenotypic traits from those of JH-2N.These suggests that JH-3N has the potential for commercialisation and application in production.The findings of this study provide a wealth of basic biology data for the hybrid ploidy breeding and population identification of hybrid grouper.

hybrid groupertriploidgrowth ratemorphological charactersmultivariate statistics

邓远、苏金枝、李振通、黎琳琳、邱弈树、丁小雨、段鹏飞、王心怡、王林娜、刘阳、马文辉、赵侠、李胜忠、田永胜

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海水养殖生物育种与可持续产出全国重点实验室 中国水产科学研究院黄海水产研究所 山东青岛 266071

新疆农业大学生命科学学院 新疆乌鲁木齐 830052

中国水产科学研究院海南创新研究院 海南三亚 572000

莱州明波水产有限公司 山东莱州 261400

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金虎杂交斑 三倍体 生长速度 形态特征 多元统计

海南省重点研发计划项目"十四五"国家重点研发计划泰山产业领军人才项目山东省重点研发计划项目财政部和农业农村部:国家现代农业产业技术体系中央公益性科研院所基本科研业务费中国水产科学研究院科研业务费

ZDYF2022XDNY243号2022YFD2400502号LJNY202109号2022LZGC016号CARS-47-G31号20603022024013号2020TD19号

2024

海洋与湖沼
中国海洋湖沼学会 中国科学院海洋研究所

海洋与湖沼

CSTPCD北大核心
影响因子:0.737
ISSN:0029-814X
年,卷(期):2024.55(5)