EFFECTS OF LXRα AGONIST ADDITION AND RNA INTERFERENCE ON LIPID METABOLISM OF JUVENILE TURBOT SCOPHTHALMUS MAXIMUS UNDER LOW SALT STRESS
The turbot(Scophthalmus maximus)is an important economically valuable fish in China's aquaculture industry.As aquaculture environments area expands to the tidal flats and inner areas,it is imperative to study the mechanisms of low-salinity adaptation in turbot to provide a reference for breeding low-salinity-tolerant varieties.This study investigates the expression of downstream lipid metabolism-related genes and changes in serum lipid metabolic products through intraperitoneal injection of the inhibitor dsRNA and the agonist T091317.Two salinity gradients(5 and 30)were set,with four treatment groups for each salinity(interference group,agonist group,physiological saline group,and blank control group),and the experimental period lasted for 72 h.The results showed that the relative expression of the LXRα gene in the liver was significantly inhibited after dsRNA injection,especially at a salinity of 30;however,the interference effect was not significant in the liver at 5 and in the intestinal groups at different salinities.After injection of the agonist,the relative expression of the LXRα gene showed an agonistic effect in different salinity groups,but the duration was short.Low salinity inhibited the expression of SREBP-1,CYP7A1,and ApoA-Ⅳ genes.When the LXRα gene was inhibited or activated,despite different expression trends,all three genes showed corresponding interference and activation effects overall.Serum biochemical results showed that low salinity significantly inhibited the levels of TG and HDL,with no significant effect on T-CHO and LDL levels.At a salinity of 30,when the LXRα gene was inhibited or activated,the TG content showed corresponding interference and activation effects.The above results indicate that intraperitoneal injection of the inhibitor dsRNA and the agonist can correspondingly lead to a decrease or increase in LXRαgene expression.Inhibition of the LXRα gene can lead to a reduction in the expression of the downstream transcription factor SREBP-1 gene,thereby inhibiting TG synthesis.Therefore,the serum triglyceride content can be further used as a phenotype for selecting low-salinity-tolerant turbot.
万方数据
维普
