STUDIES ON THE PATTERNS OF OVARY RE-DEVELOPMENT AND SCREENING THE KEY GENES AND SIGNALING PATHWAYS INITIATING RE-DEVELOPMENT OF MACROBRACHIUM ROSENBERGII
Under suitable temperature conditions,Macrobrachium rosenbergii can spawn year-round,and its multiple spawning characteristics are often used in nursery production to increase seedling yields,thereby improving broodstock utilization rates and economic benefits.To better understand the dynamic changes of the ovary re-development and to find the key genes and signaling pathways initiating re-development of M.rosenbergii,we investigated the external morphology,internal histology,and transcriptomics of M.rosenbergii ovaries at different developmental stages during re-development process.Results show that the ovary re-development cycle can be divided into five stages:oogonia accumulation,oocyte formation,oocyte maturation,re-spawning,and recovery.Through transcriptome sequencing,99.3 Gb clean data were obtained from the ovaries of five re-development stages,and 89 614 Unigenes were successfully annotated.Totally,32 288 differentially-expressed genes were identified,including 17 009 up-regulated and 15 279 down-regulated genes.The construction using the WGCNA(an R package for weighted correlation network analysis)co-expression network revealed that the expression of genes in the MEgreen module was high in Stage Ⅰ ovaries and decreased as the ovaries matured,showing an upward trend after ovulation.Using the protein-protein interaction(PPI)network,we analyzed the interactions among genes in this module,and found that both ribosome and proteasome signaling pathways,along with related genes such as ribosomal protein genes(RPL24,RPS24,RPL10a,RPS3a)and proteasome gene families(PSMC2,PSMC4,PSMC6,PSMD4),play crucial roles in initiating ovary re-development.KEGG enrichment analysis also revealed significant enrichment of the ribosome and proteasome pathways(P.adjust＜0.05).These findings lay a theoretical foundation for revealing the histomorphological characteristics of ovary re-development of M.rosenbergii and the molecular regulatory mechanisms of associated genes.
万方数据
维普
