COMPARATIVE METABOLOMICS ANALYSIS OF OVARIES OF FEMALE SHRIMP WITH DIFFERENT SPAWNING FREQUENCY IN LITOPENAEUS VANNAMEI
To explore the molecular mechanism of the difference in spawning frequency of female shrimp of Litopenaeus vannamei,the endogenous metabolites of female shrimp in the early and late stages of maturation and development were analyzed by liquid chromatography coupled with mass spectrometry(LC-MS)non-targeted metabolomics technology.Results show that 156 differential metabolites were detected in the early stage of ovarian maturation and development,mainly including lipid-related metabolites such as cholesterol,androstenedione,phosphatidylcholine(PC),docosahexaenoic acid(DHA),prostaglandin E2,and amino acids such as arginine,phenylalanine,serine,valine,and leucine,and were enriched in 128 metabolic pathways,including ovarian steroidogenesis,steroid hormone biosynthesis,biosynthesis of amino acids,and other pathways.In the late stage of ovarian maturation and development,11 differential metabolites were detected,mainly including L-carnitine,testosterone,phosphatidylcholine,and son on,and were enriched in 10 metabolic pathways,such as glycerophospholipid metabolism and ovarian steroidogenesis.In addition,the differential metabolites of the ovaries of female shrimp with different spawning frequencies were concentrated mainly in the early stage of maturation and development,and lipid and amino acid metabolism might play an important role in the speed regulation of ovarian maturation and development.This study provided a theoretical basis for the study of the internal regulatory mechanism of the difference in spawning frequency of L.vannamei,with a theoretical support to the development of reproductive regulation technology for crustaceans such as L.vannamei.