Yellow drum(Nibea albiflora)is an important marine economic fish species in China.As the scale of breeding gradually expands,the demand for new high-quality breeding germplasm becomes urgent.Unsaturated fatty acid content,as an important nutritional quality trait,is also gaining widespread attention from the researchers.The offspring of a breeding population consisting of 179 yellow drums was analyzed by whole-genome re-sequencing.As a result,2 940 251 high-quality single nucleotide polymorphism(SNP)loci were obtained.The fatty acid composition of each sample was determined,and with the traits such as n-3 long-chain polyunsaturated fatty acid content as phenotypic characteristics,a genome-wide association analysis was performed based on a mixed linear model,and the molecular markers related to the muscle fatty acid composition in yellow drum were identified.A total of 19 significantly-associated SNP loci were pinpointed,and 27 candidate genes were screened,in which the aamdc(Mth938 domain-containing protein)gene was considered in previous studies as being involved in regulating the fat synthesis process,which may serve as an important marker gene for subsequent research on the muscle fatty acid composition and related breeding studies in yellow drum.This study provided a reference for the genetic mechanism research of fish muscle fatty acid composition and also a theoretical guidance for the breeding of superior new varieties of yellow drum.
关键词
黄姑鱼/脂肪酸组成/重测序/全基因组关联分析
Key words
Nibea albiflora/fatty acid composition/re-sequencing/whole genome association analysis
维普
万方数据