血样保存时间对CD4+T淋巴细胞检测的影响分析
Influence of storage time of blood samples on detection of CD4+T lymphocyte
张姝 1边国林 1倪红霞 1高红 1许国章1
作者信息
- 1. 宁波市疾病预防控制中心,浙江宁波315010
- 折叠
摘要
目的 了解保存不同时间对全血样品定量检测T淋巴细胞的影响情况.方法 收集12份HIV感染者的抗凝全血,于4～8℃条件下保存,采用流式细胞仪、四色荧光抗体试剂和计数微球,在采血当日和采血后5d每隔24 h定期检测CD4+T淋巴细胞绝对值,并用SPSS 13.0软件分析数据.结果 与采血当日CD4T淋巴细胞定量检测结果比较,采血后24、48、72、96、120 h的检测结果平均下降了14.583、22.083、49、53.417、88个/μ(l),其中采血后24 h的结果与采血当日比较差异无统计学意义(P=0.09),其他差异均有统计学意义(P＜0.05).结论 在4～8℃保存条件下,进行T淋巴细胞定量检测的全血样品最好在采血后24 h内完成检测,检测结果较为可靠,最迟不要超过48 h,保存时间越长,CD4T淋巴细胞值下降得越多.
Abstract
Objective To understand the influence of storage time on quantitative detection of CD4+ T lymphocyte with whole blood samples. Methods Twelve whole blood samples were collected from HTV-1 infected persons and stored at 4-8^. The absolute numbers of CD4+ were detected every 24 hours from day 1 to day 6 after sampling with flow cytometer, four color fluorescence monoclonal antibody reagent and COULTER EPICS XL. The results were analyzed with SPSS 13. 0 software. Results Compared with the quantitative detection result of CD4+ on sample collection day, the average number of CD4+ declined by 14. 583/jxl 24 hours later, 22. 083/ui 48 hours later, 49/ui 72 hours later, 53.417/u,l 96 hours later and 88/(jl,1 120 hours later. The difference on the detection results between the sample collection day and 24 hours later had no statistical significance (P = 0. 09), but the differences on other detection results had statistical significance (P < 0. 05). Conclusion The detection result of CD4+ in blood samples (stored at 4 - 8 t) is reliable if the detection is completed within 24 hours or no more than 48 hours. The longer the samples are stored, the more CD4+ T lymphocyte decline
关键词
保存时间/CD4+T淋巴细胞/血样Key words
storage time/CD4+ T lymphocyte/blood sampless引用本文复制引用
出版年
2012
NETL
NSTL
维普
万方数据