Genome-wide molecular characterization analysis of rare G9P[8]-A2 genotype rotavirus in China
Objective To investigate the genome-wide molecular characteristics of group A rotavirus(RVA)JL18221140,a G9P[8]genotype in China.Methods A raw fecal specimen of G9P[8]genotype RVA(JL18221140)in Jilin province,China,was amplified by reverse transcription-polymerase chain reaction(RT-PCR)one-step method to amplify 11 gene segments,and homology and evolution analysis were carried out by DNAStar,MEGA 11.0 and other bioinformatics analysis software.Results The genotypes of 11 segments of the whole genome of JL18221140 were G9-P[8]-I1-R1-C1-M1-A2-N1-T1-E1-H1,and the NSP1 genotype showed DS-1-like characteristics.Phylogenetic analysis showed that VP7 and VP4 were clustered in lineages G9-Ⅵ and P[8]-Ⅲ,respectively.Conclusion JL18221140 is a rare G9P[8]-A2 reassortant strain among G9P[8]genotypes,which may have arisen from inter-genomic reassortment during co-infection with the G9P[8]-A1 and G2P[4]-A2 strains prevalent in China.This shows the importance of continuous monitoring of RVA and whole genome sequencing,which is conducive to understanding the genetic variation of RVAstrains in China,and provides more effective basic data for future virus prevention and control and vaccine research and development.
Group A rotavirusGenome-wideSequencingG9P[8]VariationReconfiguration
NETL
NSTL
万方数据
