Analysis on HIV-1 genotype distribution and drug resistance in Hongkou district,Shanghai,2021
Objective To understand the genotypes and drug resistance of HIV-1 in Hongkou district of Shanghai in 2021,and provide data support for establishing HIV-1 drug resistance database and adjusting prevention and treatment strategies of acquired immune deficiency syndrome in time.Methods Plasma samples of HIV-1 positive patients were collected from January to December,2021,and the Pol gene of HIV-1 was amplified by reverse transcription-nested PCR.The genotypes of HIV-1 were identified by COMET tool and phylogenetic tree,and the drug resistance was analyzed by Stanford University HIV Drug Resistance Database.Results A total of 143 Pol sequences and 10 genotypes were obtained.The HIV-1 genotypes included CRF01_AE,CRF07_BC,C,B,CRF08_BC,CRF55_01B,CRF67_01B,CRF68_01B,A,and URFs,accounting for 34.27％,36.36％,9.09％,6.29％,6.29％,2.80％,1.40％,0.70％,0.70％,and 2.10％,respectively.It was found that the rate of pretreatment HIV drug resistance(PDR)was 10.49％(15/143).The PDR rate of non-nucleoside reverse transcriptase inhibitors was 6.29％(9/143).The PDR rate of nucleoside reverse transcriptase inhibitors was 1.40％(2/143).The PDR rate of protease inhibitors(PIs)was 2.80％(4/143).The PDR rate of rilpivirine,nevirapine and efavirenz were 4.90％,3.50％and 3.50％,respectively.In addition,the rate of transmitted HIV drug resistance(TDR)was 4.20％(6/143),and the TDR mutations were detected at M46I,M184V,K103N,K101E and Y181C,which were predicted to be highly resistant to efavirenz,nevirapine,emtricitabine and lamivudine.Conclusion The genotypes of HIV-1 in Hongkou are becoming increasingly diverse.Therefore,surveillance for HIV-1 genotypes and drug-resistant strains,especially the PDR and TDR associated with non-nucleoside reverse transcriptase inhibitors,should be further strengthened.
HIV-1GenotypePretreatment drug resistanceTransmission drug resistance
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