Effects of Different Pretreatment Methods on Periplaneta americana Oil Compounds by GC-MS
Objective To analyze chemical constituents of Periplaneta americana oil extract after different treatments for its development and provide the experimental basis for the uses.Methods Using the supercritical CO2 to extract Periplaneta americana imago oil after different treatments and the samples were separated and qualitative analyzed by GC-MS-DS.Relative percentage content of the chemical constituents in the test samples was determinated by chromatographic peak area normalization method and the better treatment method of Periplaneta americana grease GC-MS analysis was determinated.Results Non-methyl esterification treatment separated and identified 13 components ofPeriplaneta americana; acid methyl esterification treatment separated and identified 26 components; alkali methyl esterification treatment separated and identified 48 components.Alkanes and fatty acid esters were the main compounds by three treatment methods,but there is great difference in the type and relative content.Conclusion Periplaneta americana oil contains a variety of compounds mainly with alkanes and fatty acids esters.It could significantly optimize the analysis results by esterification treatment before GC-MS analysis.
Periplaneta americanasupercritical CO2 extractionoil compoundsGC-MS
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