Effects of Polygonatum sibiricum Polysaccharides on Peripheral Hemogram and Gut Microbiota in Mice Irradiated by X-ray
Objective To investigate the radioprotective potential of Polygonatum sibiricum polysaccharides(PSP)against hemopoiesis injury and gut microbiome by X-ray.Methods Thirty C57BL/6J mice were randomly divided into three groups:normal group,irradiation group and PSP group.The white blood cell(WBC),red blood cell(RBC),platelet and the percentage of blood cells were analysed.The feces in the mouse intestines were collected at Days 7 post-radiation,and the 16S rRNA high-through put sequencing was used to detect alterations in the diversity and composition of gut microbiota.Results The counts of WBC at 4th,7th,10th days,and RBC at 1st,14th days after irradiation in PSP group were significantly higher than those in irradiation group(P＜0.05).PSP alleviated the irradiation-induced reduction of lymphocyte percentage and attenuated the increment of neutrophil percentage and monocyte percentage after irradiation.α diversity showed no significant differences in both irradiation group and PSP group.β diversity showed a significant shift of microbial composition in irradiated group and PSP group.PSP treatment increased the abundance of bacteria involved in material metabolism.Conclusion PSP shows obvious radio protective effects on haemopoiesis injury and alters the gut microbiota of irradiated mice,which provide a basis for developing novel radio protective agents.
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