Regulation of EMT by Jiawei Danxuan Koukang in Oral Submucous Fibrosis
Objective To investigate the regulatory effect of modified Jiawei Danxuan Koukang on epithelial-mesenchymal transition(EMT)in oral submucosal fibrosis(OSF)and its regulatory mechanismal involving the microRNA(miR)-4725-3p(MIR)/secretory curl related protein 4(SFRP4)signaling pathway.Methods OSF tissue samples and normal oral mucosa tissues were collected.Human immortalized epidermal cells(HaCaT)were stimulated with arecoline to simulate OSF model in vitro,and treated with Jiawei Danxuan Koukang.Cells of the arecoline group and the Jiawei Danxuan Koukang+coline group were intervened with MIR mimics,MIR inhibitors,and MIR negative controls(MIR NC)respectively.The expression of MIR,SFRP4,and EMT-and fibrosis-related proteins E-cadherin,Vimentin,and cytokeratin 19 were detected.The interaction between MIR and SFRP4 was detected.Results Compared with normal oral mucosa group,the expression of MIR was down-regulated and SFRP4 was up-regulated in submucosal fibrosis group.Also,MIR negatively regulated SFRP4.In cellular experiments,the OSF model was successfully established using arecoline.After treatment with Jiawei Danxuan Koukang,the expressions of MIR,cytokeratin 19 and E-cadherin were increased,while the expressions of SFRP4 and Vimentin were decreased.Compared to the Jiawei Danxuan Koukang+MIR NC treatment group,the Jiawei Danxuan Koukang+MIR inhibitor treatment group exhibited opposite trends in these indicators.Conclusion Jiawei Danxuan Koukang can inhibit epithelial-mesenchymal transition in OSF by regulating MIR/SFRP4 axis.
Jiawei Danxuan KoukangmicroRNA-4725-3psecreted frizzled-related protein 4oral submucous fibrosisepithelial-mesenchymal transition
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