基于UPLC-QE-Orbitrap-MS技术的复方四物汤化学成分表征与鉴定
Characterization and Identification of Chemical Components in Compound Siwu Tang Based on UPLC-QE Orbitrap MS Technology
贾鹏晖 1王瑞2
作者信息
- 1. 蚌埠市第三人民医院药剂科,安徽蚌埠 233000
- 2. 芜湖职业技术学院食品与生物工程学院,安徽芜湖 241000
- 折叠
摘要
目的 建立超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-QE-Orbitrap-MS),系统开展中药复方四物汤化学成分的表征与鉴定.方法 采用BEH C18色谱柱(2.1 mm × 100 mm,1.7 µm),流动相为0.1％甲酸水-乙腈,流速为0.5 mL/min,柱温为30 ℃,在正、负离子模式下采用Auto MS/MS进行数据采集.结果 通过二级质谱裂解碎片解析以及文献分析等,从复方四物汤中共鉴定或初步推导了 47种化合物,包括5种有机酸、3种酚类、12种黄酮类、6种苯丙素类、15种萜类、2种倍半萜和4种生物碱.结论 建立的UPLC-QE-Orbitrap-MS能快速、准确、较全面地鉴定复方四物汤的化学成分,为其药效物质基础研究提供参考依据.
Abstract
Objective To establish high resolution mass spectrometry with ultra-high performance liquid chromatography(UPLC-QE-Orbitrap-MS)and systematically represent and identify the chemical composition of TCM compound Siwu Tang.Methods Data acquisition by BEH C18 column(2.1 mm× 100 mm,1.7 μm),0.1％formic acid water-acetonitrile,flow rate was 0.5 mL/min,column temperature was 30℃,and Auto MS/MS in positive and negative ion mode.Results 47 compounds,including 5 organic acids,3 phenols,12 flavonoids,6 phenylpropanoids,15 terpenes,2 sesquiterpenes and 4 alkaloids were identified or preliminarily derived from the compound Siwu Tang through secondary mass spectrometry fragmentation analysis and literature analysis.Conclusion The established UPLC-QE-Orbitrap-MS can quickly,accurately and comprehensively identify the chemical components of compound Siwu Tang,providing a reference basis for the study of its pharmacological substance basis.
关键词
UPLC-QE-Orbitrap-MS/化学成分/结构鉴定/复方四物汤Key words
UPLC-QE-Orbitrap-MS/chemical composition/structure identification/compound Siwu Tang引用本文复制引用
基金项目
安徽省高等学校自然科学类研究项目(2022AH052203)
出版年
2024
NETL
NSTL
万方数据