首页|基于系统聚类和PLS回归分析的栝楼种子等级综合评判研究

基于系统聚类和PLS回归分析的栝楼种子等级综合评判研究

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  • NETL
  • NSTL
  • 万方数据
目的 建立栝楼种子等级评价模型，科学评判不同产地栝楼种子的品质。方法 以25批栝楼种子为研究对象，测定各批次种子净度、含水量、千粒重、发芽率、生活力等指标参数，并以此为依据，利用SIMCA-P 14。0软件对数据进行系统聚类分析(HCA)、偏最小二乘法-判别分析(PLS-DA)及偏最小二乘回归分析(PLS)，并建立等级预测模型。结果 根据HCA可以将25批栝楼种子样品划分为3个等级，PLS-DA表明该等级划分合理;PLS回归分析预测得到栝楼种子质量等级的回归方程为:Y=2。618+0。006X1-0。045X2-0。035X3+0。005X4(X1～X4 分别为千粒重、含水量、生活力、发芽率)，若 Y在 0。5～1。5之间，则判别为一级种子;若Y在1。5～2。5之间，则为二级种子;若Y在2。5～3。5之间，则为三级种子。结论 建立的栝楼种子等级快速评价模型评价效果较为理想，可为栝楼种子质量等级的预测和良种选育提供理论依据。
Study on Comprehensive Evaluation of the Seed Grade of Trichosanthes kirilowii Maxim.Based on Hierarchical Cluster Analysis and PLS Regression
Objective To establish the model of quality evaluation of T.kirilowii seeds and scientifically evaluate its quality grade.Methods The purity,water content,1 000-grain weight,germination rate and viability of 25 T.kirilowii seeds from different areas were determined.These results were analyzed by hierarchical cluster analysis(HCA),partial least squares discriminant analysis(PLS-DA)and partial least squares(PLS)regression analysis based on SIMCA-P 14.0 software.Furthermore,a prediction model for grading of T.kirilowii seeds was constructed by PLS regression analysis.Results The results showed that 25 batches of T.kirilowii seeds could be divided into three grades according to HCA,and was reasonable evaluating by PLS-DA.The PLS regression model for quality grade of T.kirilowii seeds was constructed as follows:grade Y=2.618+0.006X1-0.045X2-0.035X3+0.005X4(X1:1 000-grain weigh,X2:water content,X3:viability,X4:germination rate),if Y=0.5～1.5,the predicted quality was grade A,if Y=1.5～2.5,then B grade,if Y=2.5～3.5,C grade.Conclusion The mode of quality grading evaluation performed well.The study provided a theoretic basis for the quality grading prediction and the breeding of improved varieties of T.kirilowii seeds.

Trichosanthes kirilowii Maxim.seeds gradeHCAPLS-DAPLS regression

胡燕珍、张媛媛、黄斌、孟岩、陈乐、文萍

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江西省中医药研究院,江西南昌 330046

江西道地特色中药材标准化重点研究室,江西南昌 330046

栝楼 种子等级 系统聚类分析 PLS-DA PLS回归分析

江西省中医药标准化技术委员会2021年第一批标准化项目江西省中医药中青年骨干人才(第四批)培养计划江西省中医药管理局重点研究室-江西道地特色中药材标准化重点研究室财政部和农业农村部:国家现代农业产业技术体系

2021A03赣中医药科教字[2022]6号KP22103002CARS-21

2024

10.13728/j.1673-6427.2024.02.001

现代中药研究与实践
安徽中医药高等专科学校,

现代中药研究与实践

CSTPCD
影响因子：0.409
ISSN：1673-6427
年,卷(期)：2024.38(2)