Transcriptome Sequencing and Bioinformatics Analysis of Schisandra chinensis(Turcz.)Baill in Daxing'an Mountains
Objective To obtain the transcriptome information of Daxing'an Mountains Wuweizi seeds.Methods After transcriptome sequencing,the Unigene sequence was obtained,and the Unigene was compared with the seven functional database annotations,SSR analysis and CDS prediction to obtain the information of the Wuweizi transcriptome.Results A total of 19.41G of Clean Data was obtained from transcriptome sequencing.The effective data volume of each sample was distributed at 6.26～6.72 G,Q30 base was distributed at 95.47％～96.07％,and the average GC content was 47.73％.Unigene 23 149 strips.Daxing'an Mountains Schisandra chinensis(Turcz.)Baill and Nelumbo nucifera had the highest sequence similarit.Due to annotations to the NR database,13 531 genes were annotated to the Swissprot database,4 228 genes were annotated to the KEGG Database,10 821 genes were annotated to the KOG database of adjacent classes of eukaryotic proteins,and 11 727(50.66％)genes were annotated to the GO Database.It was predicted that 7 468 SSRs,20 468 CDS sequences were predicted.Conclusion The research results enrich the transcriptome data of Daxing'an Mountains Schisandra chinensis(Turcz.)Baill,provide a reference for in-depth study of the biological characteristics and molecular mechanisms of Daxing'an Mountains Schisandra chinensis(Turcz.)Baill,and also conducive to the development,protection and utilization of its resources.
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