Study on Lipid-lowering Effects and Mechanisms of Lotus Leaf Water Extract Using an in vitro Model of Nonalcoholic Fatty Liver Disease
Objective To investigate the effect of Lotus leaf water extract(LLWE),nuciferine(NF),pronuciferine(PF)and dehydronuciferine(DF)on lipid metabolism pathway AMPK-SREBP1-ACC1.Methods The UPLC-QTOF/MS was used to identify chemical constituents of LLWE.The HepG2 cellular model of NAFLD was induced by free fatty acids.The lipid accumulation was detected by BODIPY staining,and the Western Blotting determined the protein expression levels of AMPK,SREBP1 and ACC1.Results A total of 15 compounds were identified from LLWE by UPLC-QTOF/MS,mainly including alkaloids and flavonoids.The intervention of either NF or PF significantly reduced lipid accumulation with SREBP1 and p-ACC1 decreased,but increased p-AMPK in FFA-induced HepG2 cells.Additionally,the combination of either NF or PF with LLWE significantly enhanced the lipid-lowering effects of NF or PF alone.Conclusion NF and PF are main lipid-lowering cehmicals in LLWE,which might act through regulating the AMPK-SREBP1-ACC1 pathway.
Lotus leaf water extractnuciferinepronuciferinenonalcoholic fatty liver diseaseAMPK/SREBP1/ACC1 pathway
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