Before and After Electron Beam Irradiation of Thesii Herba Based on UPLC Characteristic Chromatogram and Quantitative Index Components
Objective To explore the effects of electron beam irradiation sterilization on the characteristic spectra and various index components of Thesii Herba from three different regions,and provide a basis for evaluating the quality of Irradiated Thesii Herba.Methods With the content of 5-O-ferulic quinic acid,4-O-ferulic quinic acid,thymosin Ⅰ and kaempferol 3-O-(2"-O-α-rhamsyl-6"-O-malonyl)-β-glucoside as the evaluation indexes.The influence of electron beam irradiation with different irradiation doses(1,3,6,10,15,20 kGy)on the quality of Thesii Herba was evaluated through the detection of the index component content and characteristic spectra,combined with principal component analysis(PCA)and cluster analysis(CA).Results 5 common peaks were calibrated in the characteristic spectra of different irradiation doses of Thesii Herba.The similarity between the irradiated and non-irradiated samples was greater than 0.99,and the PCA and CA results showed that there was no significant difference in the characteristic peak area of each irradiation dose sample.The overall quality of Thesii Herba at different irradiation doses was relatively stable.The content determination results showed that after irradiation,the content of various indicator components in Thesii Herba decreased,but there was no significant correlation with the irradiation dose.The overall fluctuation range was small,especially for the phenolic acid components 5-O-ferulic quinic acid and 4-O-ferulic quinic acid,which showed a decreasing trend with the increase of irradiation dose and were the active components greatly affected by irradiation.Conclusion After being irradiated with different doses of electron beam,there is no significant change in the characteristic spectra of Thesii Herba.The content of each indicator component slightly decreased,and the overall reduction rate is within an acceptable range.Radiation sterilization is applicable to the sterilization of Thesii Herba medicinal materials from 3 different regions within the range of irradiation doses studied.
Thesii Herbaelectron beam irradiationUPLC specific chromatogramcontent determinationstoicheiometry
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