Epimedium Improves Cognitive Function in Alzheimer's Disease Mice by Promoting Hippocampal Neural Regeneration
Objective To study the effect of Epimedium on improving cognitive function via promoting hippocampal neural regeneration in Alzheimer's disease(AD)model mice.Methods 6-month-old APP/PS1 double transgenic mice were randomly divided into model group,Epimedium low,medium and high dose groups and positive control drug group.Mice were administered orally once a day for 28 consecutive days.Using Nesting and Morris water maze experiments to test the daily living,learning and memory abilities of the mice;Nissl staining and immunofluorescence were used to detect the number of Nissl bodies,mature neurons,neural stem cells(NSCs)and the activation of Astrocyte in hippocampus;Transmission electron microscopy was used to detect neuronal ultrastructure.Hippocampal NSCs of AD mice were cultivated in vitro and their proliferation and differentiation were detected.Results Compared with the model group,the learning,memory and living abilities of the Epimedium group mice were significantly improved.The number of Nissl body and mature neuron in the hippocampus were significantly increased.The damage to neuronal mitochondria was significantly reduced,and both the number of synapses and the thickness of postsynaptic dense matter increased significantly,while the number of activated Astrocyte decreased significantly,demonstrating that Epimedium inhibits the immune inflammatory reaction in the brain,and reduced neuronal damage.At the same time,it also significantly increased the number of hippocampal NSCs in the brain.In vitro experiments also confirmed that Epimedium could promote the proliferation of AD-NSCs and induce their differentiation into neurons.Conclusion Epimedium can improve cognitive function in AD mice by protecting brain tissue and activating endogenous NSCs to promote hippocampal neural regeneration.
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