摘要
目的 研究栽培黄精种质资源表型性状的多样性和变异特征,建立黄精表型性状综合评价体系.方法 以360份栽培黄精种质为试验材料,选取16个表型性状进行测定,并对测定数据开展多样性分析、相关性分析、聚类分析和主成分分析等多元统计分析,建立黄精表型性状综合性评价体系.结果 栽培黄精表型变异主要存在于居群内.黄精表型性状遗传多样性丰富,其中,叶长、果实横径、根茎粗、果形指数、根茎最粗、株高、果实纵径等性状变异程度较高.主成分分析表明,株高、地径、根鲜重、根茎最粗、根茎粗、叶数、叶长、叶宽、果实横径等9个表型性状含有丰富的遗传信息以及具有选择潜力,能保留大部分的表型遗传多样性信息,可优先作为栽培黄精评价的重要指标.聚类分析将黄精分为3个类群.结论 研究建立了黄精表型性状综合评价体系,为黄精优良种质筛选提供参考.
Abstract
Objective To clarify the diversity and variation characteristics of phenotypic traits in cultivated P.sibiricum germplasm resources,and to establish a comprehensive evaluation system for phenotypic traits.Methods A total of 360 cultivated P.sibiricum germplasm was selected from 24 batches as experimental materials,and 16 phenotypic traits were selected for determination,and multivariate statistical analyses such as diversity analysis,correlation analysis,cluster analysis and principal component analysis were carried out to establish a comprehensive evaluation system for phenotypic traits of P sibiricum.Results The phenotypic variation of cultivated P.sibiricum mainly existed in populations.The genetic diversity of phenotypic traits of was abundant,and the variation of leaf length,fruit transverse diameter,root diameter,fruit shape index,root thickness,plant height,and fruit longitudinal diameter was relatively high.Principal component analysis(PCA)showed that 9 phenotypic traits,including plant height,ground diameter,root fresh weight,root thickness,root thickness,leaf number,leaf length,leaf width,and fruit transverse diameter,contained abundant genetic information and had the potential to select,and could retain most of the phenotypic genetic diversity information,which could be preferentially used as important indicators for the evaluation of cultivated P sibiricum Delar.ex Redoute.Cluster analysis divided Polygonatum into 3 groups.Conclusion A comprehensive evaluation system for phenotypic traits of P.sibiricum is established,which provide a reference for the screening of excellent germplasm of P.sibiricum.
基金项目
辽宁省卫健委"辽宁省道地药材标准体系建立"项目(2022003)
辽宁省"兴辽英才计划"教学名师项目(XLYC2211011)
辽宁省农村科技特派行动计划项目--辽宁省喀左县道地药材科技特派团(2024JH5/10400152)
维普
万方数据