Study on the Difference of Epimedium in Different Species Based on Plant Metabolomics
Objective To study the overall chemical composition differences of Epimedium from the perspectives of origin and growing areas.Methods Liquid chromatography-tandem mass spectrometry(LC-MS/MS)was used to analyze the metabonomics of four Epimedium species,and the differential components were screened.Meanwhile,the contents of total flavonol glycosides and total flavonoids were determined by liquid chromatography and ultraviolet spectrophotometry,and to study the content differences of index components in different species of Epimedium.Results There were certain differences in the contents of total flavonol glycosides and total flavonoids among different origins of Epimedium.The overall chemical compositions of Epimedium from different regions showed differences,and the origin and growing areas were corresponding.There were 65 differential components between Epimedium brevicomu Maxim.and Epimedium koreanum Nakai.There were 125 differential components between Epimedium brevicornu Maxim.and Epimedium pubescens Maxim..There were 109 differential components between Epimedium brevicornu Maxim.and Epimedium sagittatum(Sieb.et Zucc.)Maxim..Among them,there were 19 differential components commonly.Conclusion The differences of chemical constituents of different species Epimedium are effectively revealed,which provide a reference for the identification of the origin of Epimedium and beneficial to the rational utilization of Epimedium resources.