Analysis of the Effect of Yiqi Huoxue Prescription on the Diversity of Intestinal Flora in Rats with Cerebral Ischemia Injury Based on 16S rDNA Sequencing Technology
Objective To investigate the effects of Yiqi Huoxue prescription on the diversity of intestinal flora in rats with cerebral ischaemia injury based on 16S rDNA sequencing technology and explore the mechanism of anti-ischemic injury of the formula from the perspective of the"brain-gut"axis.Methods 36 male SD rats were randomly divided into 6 groups,with 6 rats in each group,namely sham operation group,model group,nimodipine group and high,medium and low dose groups of Yiqi Huoxue prescription.After 14 d of continuous gavage,a model of acute cerebral ischaemic injury was established using the bilateral common carotid artery ligation method.The pathological changes of brain tissue were observed by hematoxylin-eosin(HE)staining,and IL-6 and TNF-α in serum were detected by ELISA.Colonic contents were collected from each group of rats,and changes in faecal intestinal flora were detected by 16S rDNA sequencing.Results Different dosage groups of Yiqi Huoxue prescription could obviously improve the degree of cerebral ischemia injury,and there was a dose-effect correlation.Compared with the sham operation group,the serum levels of IL-6 and TNF-α in the model group increased significantly(P＜0.05).Compared with the sham-operated group,the serum levels of the inflammatory factors IL-6 and TNF-α were significantly higher in the model group of rats(P＜0.05);they were significantly lower in the high-dose group of Yiqi Huoxue prescription after the intervention(P＜0.05,P＜0.01).The results of 16S rDNA sequencing showed that Yiqi Huoxue prescription could regulate the structure of intestinal flora in rats with cerebral ischaemia injury,and increase Chaol,Observed OTUs,Simpson and Shannon indices in rats,and the results of Beta Diversity Analysis showed that all the samples could be obviously divided into three groups with good clustering.Yiqi Huoxue prescription could significantly increase the abundance of Firmicutes and Patescibacteria(P＜0.05),significantly decreased the abundance of Verrucomicrobia(P＜0.05),and significantly increase the relative abundance of Lachnospiraceae_NK4A136_group,Firmicutes_unclassified,and Alloprevotella(P＜0.05),significantly down-regulated the abundance of Bacteroides,Akkermansia,Clostridium_sensu_stricto_1,Romboutsia,Veillonella and Bifidobacterium(P＜0.05).Conclusion Yiqi Huoxue prescription has a protective effect on the brain tissue of rats with cerebral ischaemia injury,and its mechanism of action may be related to the reduction of serum inflammation and the regulation of the microbial composition,structure and community abundance of the intestinal flora,which further verifies the theory of the"brain-gut"axis.
万方数据
维普
