Investigating the Cardioprotective Properties of Different Crude Extracts and Components Derived from Apocynum venetum L.Leaves against Drug-Induced Cardiac Injury in Zebrafish
Objective To assess the protective efficacy of crude extracts from Apocynum venetum L.leaves and its individual components against drug-induced cardiac injury in zebrafiish.Methods Initially,the LC50 values were assessed employing wild-type AB zebrafish.Subsequently,the zebrafish cardiac injury model was induced through the utilization of verapamil hydrochloride,and the pericardial edema and venous congestion in zebrafish were examined subsequent to the administration of various crude extracts and principal components.Results At a safe dosage,the Fr.n-BuOH,Fr.n-BuOH-40％and Fr.H2O extracts derived from Apocynum venetum L.leaves demonstrated superior cardioprotective properties against zebrafish cardiac injury compared to other crude extracts.Furthermore,the protective effect of the principal constituent,hyperoside,exhibited a significant dose-dependent relationship and was marginally more potent than isoquercitrin.Conclusion The leaves of Apocynum venetum L.and its primary constituents exhibit a protective effect against cardiac injury in zebrafish,thus necessitating further comprehensive investigations into the underlying mechanisms.
万方数据
维普
