Isolation,Purification and Anti-inflammatory Effect of Salt Processed Anemarrhenae Rhizoma Polysaccharide on RAW264.7 Cells in vitro
Objective To isolate and purify the purified polysaccharide of Anemarrhena asphodeloides(SAAP-A-1)from the crude polysaccharide of salt-processed anemarrhena asphodeloides,and to study its structure and anti-inflammatory activity.Methods The polysaccharide of SAAP was extracted by water extraction and alcohol precipitation method,and the polysaccharide SAAP-A-1 was purified by anion and cation exchange and DEAE-52 cellulose resin.The structure of SAAP-A-1 was analyzed by HPLC,PMP pre-column derivatization ultra-high performance liquid chromatography,FT-IR analysis,UV analysis,and SEM.The effect of SAAP-A-1 on the proliferation rate of mouse macrophage RAW264.7 was detected by the CCK-8 method.The contents of IL-1β,TNF-α and IL-6 in the supernatant of RAW264.7 cells were detected by ELISA.The mRNA levels of IL-1β,TNF-α,IL-4,IL-10 and iNOS were measured by RT-PCR.Results SAAP-A-1 was a homogeneous polysaccharide with a molecular weight of 3.78×105 Da.The main chain of SAAP-A-1 was mainly composed of mannose,galactose,arabinose and xylose.In vitro study showed that SAAP-A-1 could promote the proliferation of RAW264.7 macrophages,reduce the content of inflammatory factors TNF-α,IL-6,IL-1β,NO,and the expression of TNF-α,IL-1β,iNOS mRNA.It also increased the expression of anti-inflammatory mediators IL-4 and IL-10 mRNA.Conclusion SAAP-A-1 is a homogeneous polysaccharide isolated from the crude polysaccharide of Anemarrhenae Rhizoma for the first time and has application value in anti-inflammatory drugs.
Salt processed Anemarrhenae Rhizomapolysaccharidestructure analysisanti-inflammatory activity
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维普
